Part:BBa_K3498004

Inducible p3B5C promoter

Sensitive promoter against protocatechuic acid and presents its maximum induction in the presence of 1000mM RPU 1.6 of vanillinic acid with a leak of 4.5x10-3 RPU in absence of induction(Meyer, A. J et. al 2019) this part needs its regulatory protein (BBa_K3317005)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

AbrB protein inhibits B. subtilis sporulation. The AbrB protein of the spore-forming bacterium Bacillus subtilis is a repressor of numerous that is present in vegetatively growing cells and is inactivated or eliminated by the action of Spo0A at the end of the exponential phase of growth; AbrB is a DNA-binding protein that acts by repressing target genes (Banse et al., 2008) . This part can be used for inhibiting biofilm and sporulation formation in B. subtilis, it affects the production by binding into DNA and blocking transcription. It affects more than +100 genes as it is a major regulator (Zhu & Stülke, 2017).