Coding
cI 434

Part:BBa_C0052

Designed by: Maia Mahoney   Group: Antiquity   (2003-01-31)
Revision as of 01:49, 30 April 2006 by Registry (Talk | contribs)


cI repressor from phage 434 (+LVA)

The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.


Usage and Biology

434 cI is a member of the lamboid cI protein family.

The coding sequence has been modified from wild type cI to contain an LVA tag,

two TAA stop codons, and its EcoRI recognition site has been mutated out

by changing base 36 from T to C to be BioBrick-compatible.


Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

directionForward
functionRep
ligandsRecA?
proteinCI 434-LVA
swissproP16117
tagLVA
[edit]
Categories
//cds
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
directionForward
functionRep
ligandsRecA?
proteinCI 434-LVA
swissproP16117
tagLVA