Coding
Part:BBa_K3610014:Design
Designed by: Jonas Sebastian Trottmann Group: iGEM20_UZurich (2020-09-13)
LargeBit NanoLuc - codon optimized for S. cerevisiae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 139
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.
Source
The sequence was taken of Part:BBa_K3168002 from the iGEM registry and then codon optimized with SnapGene.