Coding

Part:BBa_K3610006

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-08-30)
Revision as of 13:57, 8 September 2020 by Jtrott (Talk | contribs)


EFR from A. thaliana - without signal sequence

This part is the EFR receptor from A. thaliana. It is similar to Part:BBa K3610005 but it lacks the signal sequence and is therefore not in frame. It needs to be expressed with a signal sequence. Either with the native signal sequence or with a signal sequence from another organism.

Usage and Biology

Does this work?

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2683
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2683
    Illegal BglII site found at 2348
    Illegal BglII site found at 2376
    Illegal BglII site found at 2919
    Illegal XhoI site found at 2372
    Illegal XhoI site found at 2962
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
    Illegal AgeI site found at 186
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None