Coding

Part:BBa_K3447000:Design

Designed by: Jiahao Yu, Xinyue Zhang, Yu Ma   Group: iGEM20_Jilin_China   (2020-09-03)
Revision as of 14:26, 4 September 2020 by Alswey (Talk | contribs) (→‎Source)


luxI, an autoinducer synthetase for AHL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

abaaba


Source

We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.
This gene is derived from Escherichia coli (strain: A2363), we get the genomic sequence by NCBI.
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164
We ordered the part DNA from a synthesis company.

References