Coding

Part:BBa_K3447000

Designed by: Jiahao Yu, Xinyue Zhang, Yu Ma   Group: iGEM20_Jilin_China   (2020-09-03)
Revision as of 14:22, 4 September 2020 by Alswey (Talk | contribs)


luxI, an autoinducer synthetase for AHL

Colicin V is a non-ribosome-encoded antibacterial peptide produced by many E. coli strains and other members of the Enterobacteriaceae family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of Gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets Cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of E. coli.

The cvaA and cvaB genes encode components of a dedicated secretion system required for extracellular secretion of colistin. The CvaB protein encoded by the cvaB gene is the central membrane transporter of the bacteriocin V secretion system in Escherichia coli. CvaB, together with CvaA and TolC proteins, direct the secretion of colicin V through the cleavage of the bisglycine leader peptide. The sequence alignment shows that CvaB protein belongs to the ABC superfamily. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None