Coding

Part:BBa_K3447002

Designed by: Fei Cao, Yu Ma   Group: iGEM20_Jilin_China   (2020-09-04)
Revision as of 14:06, 4 September 2020 by Alswey (Talk | contribs)


xynD, which produces xylanase

Colicin V is a non-ribosome-encoded antibacterial peptide produced by many E. coli strains and other members of the Enterobacteriaceae family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets Cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of E. coli. The cvaC gene produces colicin V, which is also called ColV, a type of bacteriocin secreted by E. coli. Besides, it has a killing effect on other closely related bacteria that cannot secrete specific colicin immunity protein, which is synthesized by cvi gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 712


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Categories
Parameters
None