Coding

Part:BBa_K3610002

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-01)
Revision as of 14:20, 1 September 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


BAK1 from Arabidopsis thaliana without signal sequence

This is a modification of the part BBa_K3610004, which entails the sequence for the plant cell surface receptor BAK1 from A. thaliana. This modificated part does not contain the N-terminal signal sequence necessary for the protein to localize at the cell surface in A. thaliana. This allows the insertion of any signal sequence from other organisms. This part is thus better suited for the expression in some organisms that do not recognize the original signal peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None