Coding

Part:BBa_K3610006:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-08-30)
Revision as of 15:45, 30 August 2020 by Registry (Talk | contribs)

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EFR from A. thaliana - without signal sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2683
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2683
    Illegal BglII site found at 2348
    Illegal BglII site found at 2376
    Illegal BglII site found at 2919
    Illegal XhoI site found at 2372
    Illegal XhoI site found at 2962
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
    Illegal AgeI site found at 186
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lenght needed to allow in-frame translation and the encoded amino acids have to be small ones.


Source

There is no natural counterpart for this sequence.

References