Coding

Part:BBa_K3610004:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-08-24)
Revision as of 11:59, 24 August 2020 by Registry (Talk | contribs)

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EFR ectodomain - codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 1012
    Illegal NheI site found at 1930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mustn't contain BsaI restriction sites.


Source

Cyril Zipfel Lab

References