Composite

Part:BBa_K3458003:Design

Designed by: Jiahua Zou   Group: iGEM20_GDSYZX   (2020-08-14)
Revision as of 07:58, 17 August 2020 by Kawah (Talk | contribs) (→‎References)

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35S Promoter + HQT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 191


Design Notes

  • The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of Oryza sativa L.. The promoter GluD-1 (BBa_K3458002) that we plan to use with specific expression characteristics in Oryza sativa L. endosperm cannot drive HQT express in rice protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT composite part for comparison with the GluD-1 promoter + HQT part (BBa_K3458004) .

Source

  • The HQT gene we used comes from the genome of Lonicera japonica (GeneBank: JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..The HQT was synthesized in TIANYI HUIYUAN Company.
  • The 35s promoter (BBa_K414002) has been registered by the iGEM10_Nevada team.

References

  • Peng X, Li W, Wang W, et al. Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in Lonicera japonica[J]. Planta Med., 2010,76(16):1921-1926.