Regulatory

Part:BBa_K3458002:Design

Designed by: Jiahua Zou   Group: iGEM20_GDSYZX   (2020-08-14)
Revision as of 03:08, 17 August 2020 by Kawah (Talk | contribs) (→‎Design Notes)


GluD-1 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 225


Design Notes

In order to meet the assembly requirements of biobrick, our team checked the predicted cis-elements and modified the illegal restriction sites of non-critical parts. In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT (BBa_K3458004) and 35S::HQT (BBa_K3458003).

Source

References