Regulatory

Part:BBa_K3458002:Design

Designed by: Jiahua Zou   Group: iGEM20_GDSYZX   (2020-08-14)
Revision as of 03:06, 17 August 2020 by Kawah (Talk | contribs) (→‎Design Notes)


GluD-1 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 225


Design Notes

The GluD-1 promoter is derived from the rice genome and has a full length of 1.6 kb. Since studies have shown that the 0.2kb promoter can specifically express foreign genes in rice endosperm, and the 1.2kb efficiency is the highest, our team chose the 1.2kb GluD-1 promoter. In order to meet the assembly requirements of biobrick, our team checked the predicted cis-elements and modified the illegal restriction sites of non-critical parts.

Source

References