Coding

Part:BBa_K3458001:Design

Designed by: Jiahua Zou   Group: iGEM20_GDSYZX   (2020-08-14)
Revision as of 02:25, 17 August 2020 by Kawah (Talk | contribs) (→‎Design Notes)


HQT(Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl Transferase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • We synthesized this DNA sample through TIANYI HUIYUAN Company.

In order to have a better expression in Oryza sativa L., we have carried out codon optimization according to the codon preference of Oryza sativa L..

  • In order to meet the assembly requirements, we removed the illegal restriction site.
  • In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT (BBa_K3458004) and 35S::HQT (BBa_K3458003). (please see their registration page for more information)

Source

References