Part:BBa_K3458002:Design
HQT(Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl Transferase)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We synthesized this DNA sample through TIANYI HUIYUAN Company. In order to have a better expression in Oryza sativa L., we have carried out codon optimization according to the codon preference of Oryza sativa L.. In order to meet the assembly requirements, we removed the illegal restriction site. In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT (BBa_K3458004) and 35S::HQT (BBa_K3458003). (For more information, please see their registration page)
Source
The HQT gene we used comes from the genome of Lonicera japonica (GeneBank: JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..Then we synthesized it at TIANYI HUIYUAN Company.