DNA

Part:BBa_K3458000

Designed by: Jiahua Zou   Group: iGEM20_GDSYZX   (2020-08-13)
Revision as of 13:08, 14 August 2020 by Kawah (Talk | contribs)


HQT(Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl Transferase)

The flower buds of Lonicera japonica are widely used in Chinese medicine for their anti-inflam�matory properties. The reason why L. japonica has potent and significant effects is that it con�tains various active components, especially chlorogenic acid (CGA). This is a hydroxycinnamoyl-CoA quinate hy�droxycinnamoyl transferase (HQT) gene encoding a protein of 439 amino acids from L. japonica. 图片: ys3IS4Xy0b5rEAbE.png The biosynthetic route of synthesizing CGA from caffeoyl-CoA and quinate using hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) has been reported as the most important way for plants to synthesize CGA. And the result of recent study also showed that tissue distribution of HQT was in accordance with the pattern of CGA content. Our team aims to increase the content of chlorogenic acid in rice to make rice health product effect. In order to increase the expression level of chlorogenic acid in Oryza sativa L, our team optimized the codon of the HQT gene of Lonicera japonica and tested the expression level in the rice expression system.Our work is of significance for fur�ther studies concerned with strengthening the ef�ficacy of Oryza sativa L. by means of improved CGA content. (You can find more related information on our wiki)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None