Protein_Domain
SP-Lipocal
Part:BBa_K157036
Designed by: iGEM Team Freiburg 2008 Group: iGEM08_Freiburg (2008-10-26)
SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus.
Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005.
For further information see the description of the parts mentioned above.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 335
Illegal BamHI site found at 655 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 1042 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 12
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Categories
Parameters
//function/reporter/fluorescence
//proteindomain/internal
//proteindomain/internal
None |