Composite

Part:BBa_K3634021

Designed by: Laurence Seeley   Group: iGEM20_St_Andrews   (2020-08-09)
Revision as of 14:04, 9 August 2020 by Ls268 (Talk | contribs)


LacI-Controlled CviJI Endonuclease (+ ssrA deg. tag)

The mutant lac promoter PL8-UV5 will regulate the intracellular concentration of R.CviJI through independent lac repressor binding to the operator sites. The ssrA degradation tag AANDENYADAS will prevent unwanted leaky expression of the endonuclease from degrading the plasmid sequences when still required.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 441
    Illegal AgeI site found at 584
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None