Coding

Part:BBa_K3634015:Design

Designed by: Laurence Seeley   Group: iGEM20_St_Andrews   (2020-08-08)
Revision as of 11:39, 8 August 2020 by Ls268 (Talk | contribs) (→‎Design Notes)


LacI + mf-Lon Degradation Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for lacI was taken from BBa_K1695000 with the 3' tga stop codon removed and the initiation start codon atg replaced with the native gtg start codon to improve protein expression efficiency. BBa_K2333001, the strong mf-Lon degradation tag taken from Mesoplasma florum, was then directly attached to the 3' end of the lacI gene to give the in silico fusion product.

Source

LacI is found natively in E.coli and the initial sequence used here was taken from BBa_K1695000 (see design considerations). The strong mf-Lon degradation tag is native to Mesoplasma florum and the sequence taken from BBa_K2333001.

References