Terminator
NLS+Term

Part:BBa_K165018

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-26)
Revision as of 17:31, 27 October 2008 by Jszymans (Talk | contribs)

SV40 nuclear localization sequence + ADH1 transcription terminator

The SV40 nuclear localization sequence can be added to any coding region to localize its expression to the nucleus in yeast. This is useful for building synthetic transcription factors. In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None