Composite

Part:BBa_K3431015

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-07-23)
Revision as of 04:16, 24 July 2020 by Registry (Talk | contribs)

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T7 Promoter + RBS + Thermotoga maritima Invertase + T7 Terminator

This is a protein generator that expresses a hydrolytic protein - Beta-Fructosidase (Invertase, BBa_K3431000). We added T7 Promoter (BBa_I719005) and RBS (BBa_B0034) upstream as well as T7 terminator (BBa_K731721) downstream of Beta-Fructosidase to insure its ability of transcription and translation in PURExpress in vitro Protein Synthesis Kit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1127
    Illegal BamHI site found at 1257
    Illegal XhoI site found at 1328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 928
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 459


[edit]
Categories
Parameters
None