Device

Part:BBa_J119450

Designed by: Jacob Oyler and Ali Tauchen   Group: Eckdahl Lab   (2020-07-01)
Revision as of 19:24, 1 July 2020 by Eckdahl (Talk | contribs)

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R Clone of Schistosome Ribozyme

The R Clone of Schistosome Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It has a four nucleotide (nt) base change from the Schistosome Ribozyme (J119447) in two locations. At nt 18-21, the original ribozyme sequence of GGGC was edited to CCCC. At nt 61-64, the original ribozyme sequence of GAGA was edited to GGGG. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence for this ribozyme is found in Computational Design and Biosensor Application of Small Molecule-Sensing Allosteric Ribozymes (https://pubs.acs.org/doi/abs/10.1021/bm400299a).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None