Device

Part:BBa_J119449

Designed by: Ali Tauchen   Group: Eckdahl Lab   (2020-07-01)
Revision as of 19:00, 1 July 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


N Clone of Mammal Ribozyme

The N Clone of Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It has a five nucleotide base change from the Mammal Ribozyme (J119446). It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence for the original Mammal Ribozyme is found in Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq (https://www.nature.com/articles/s41467-019-12334-y).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None