Part:BBa_K3515012

Parathyroid Hormone Receptor with cysteine modification(s) to bind to a biosensor.

The parathyroid hormone receptor (PTHR) selectively binds parathyroid hormone (PTH) in its N- terminal extracellular domain. A fluorophore may be attached to the N- terminal domain as well to create a FRET signal. This makes it a distinguishable candidate for PTH monitoring using fluorescence resonance energy transfer (FRET). Coupling this protein with a supply of PTH bound to a second fluorophore can create a competition assay, where PTH and PTH-fluorophore compete with one another for PTHR. PTH detection is vital as PTH directly regulates renal function and is also a primary regulator of calcium levels. PTH is also a crucial biomarker used in clinical medicine for tracking the progression and status of Chronic Kidney Disease (CKD) patients. As such a biosensor for PTH tracking may be of great interest to patients and clinicians. This part includes the N- terminal extracellular domain of PTHR with a cysteine residue that will bind cysteine linker arms and be used for biosensor immobilization allowing the detection of PTH.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 110
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 26
  
• 1000
  COMPATIBLE WITH RFC[1000]