Plasmid
GSH1pr

Part:BBa_K3600003

Designed by: Sven Spoerri, Yasmine Koubaa   Group: iGEM20_EPFL   (2020-06-14)
Revision as of 18:26, 14 June 2020 by Sspoerri (Talk | contribs) (→‎Usage and Biology)


GSH1 promoter part plasmid

This part contains the promoter of the gene GSH1 from S. Cerevisiae.

Usage and Biology

  • activator: Yap1p
  • GSH1 gene encodes gamma-glutamylcysteine synthetase.1
  • The promoter GSH1 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to oxidative stress.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 975
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 563
    Illegal BsaI site found at 972
    Illegal BsaI.rc site found at 1084


Measurements

References

[1] Wu, A. L. & Moye-Rowley, W. S. GSH1, which encodes gamma-glutamylcysteine synthetase, is a target gene for yAP-1 transcriptional regulation. Molecular and Cellular Biology 14, 5832–5839 (1994).

[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None