Plasmid
TRX2pr

Part:BBa_K3600001

Designed by: Sven Spoerri, Yasmine Koubaa   Group: iGEM20_EPFL   (2020-06-11)
Revision as of 18:04, 14 June 2020 by Yasmine koubaa (Talk | contribs) (→‎References)


TRX2 promoter part plasmid

This part contains the promoter of the gene TRX2 from S. Cerevisiae.

Usage and Biology

  • activator: Yap1p
  • TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.1
  • The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to oxidative stress.
  • The binding site for Yap1p, in the promoter TXR2, is TTACTAA.1
  • The yeast toolkit for modular assembly 2 was used to assemble transcriptional units. This promoter was used as a part of type II.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 568
    Illegal BsaI.rc site found at 1089


Measurements

References

[1] Kuge, S. & Jones, N. YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides. The EMBO Journal 13, 655–664 (1994).

[2] Martínez-Pastor, M. T. et al. The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). The EMBO Journal 15, 2227–2235 (1996).

[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None