Part:BBa_K3600000

HSP12 promoter part plasmid

This part contains the promoter of HSP12, a heat-shock protein in Saccharomyces Cerevisiae that is activated by the high-osmolarity glycerol (HOG) pathway.¹

Usage and Biology

• activators: MSN2/4
• HSP12 gene encodes Saccharomyces Cerevisiae's small heat shock proteins.¹
• The promoter HSP12 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to a stress.
• The yeast toolkit for modular assembly ³ was used to assemble transcriptional units. This promoter was used as a part of type II.
• The induction of gene transcription is mediated by stress response elements STRE.²

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 308
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 326
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 308
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 308
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 57
  Illegal BsaI.rc site found at 578
  

References

[1] Varela, J. C., Praekelt, U. M., Meacock, P. A., Planta, R. J. & Mager, W. H. The Saccharomyces cerevisiae HSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A. Molecular and Cellular Biology 15, 6232–6245 (1995).

[2] Martínez-Pastor, M. T. et al. The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). The EMBO Journal 15, 2227–2235 (1996).

[3] Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).