Plasmid
YCF1pr

Part:BBa_K3600002:Design

Designed by: Sven Spoerri, Yasmine Koubaa   Group: iGEM20_EPFL   (2020-06-14)
Revision as of 17:40, 14 June 2020 by Registry (Talk | contribs)

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YCF1 promoter part plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
    Illegal NotI site found at 923
    Illegal NotI site found at 2829
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2412
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal BglII site found at 1280
    Illegal BglII site found at 2283
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
    Illegal NgoMIV site found at 2767
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References