Plasmid
HSP12pr

Part:BBa_K3600000

Designed by: Sven Spoerri, Yasmine Koubaa   Group: iGEM20_EPFL   (2020-06-09)
Revision as of 14:48, 11 June 2020 by Yasmine koubaa (Talk | contribs) (→‎Usage and Biology)


HSP12 promoter part plasmid

This part contains the promoter of HSP12, a heat-shock protein in Saccharomyces Cerevisiae that is activated by the high-osmolarity glycerol (HOG) pathway.1

Usage and Biology

  • activators: MSN2/4
  • HSP12 gene encodes Saccharomyces Cerevisiae's small heat shock proteins.
  • We choosed to work with the promoter HSP12 in order to express fluorescent protein [ref] when the S.cervisiae is exposed to a stress.
  • The induction of gene transcription is mediated by stress response elements STRE.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 308
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 326
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 308
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 308
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 57
    Illegal BsaI.rc site found at 578


Measurements

this is the biology

References

[1] Varela, J. C., Praekelt, U. M., Meacock, P. A., Planta, R. J. & Mager, W. H. The Saccharomyces cerevisiae HSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A. Molecular and Cellular Biology 15, 6232–6245 (1995).

[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None