Coding

Part:BBa_K3515004:Design

Designed by: Kody Klupt, Sono Khan   Group: iGEM20_Queens_Canada   (2020-05-22)
Revision as of 18:20, 22 May 2020 by Kody klupt (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Potassium binding protein with cysteine modification(s) to bind to a biosensor.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
    Illegal AgeI site found at 112
    Illegal AgeI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added a cysteine for immobilization (A26C) opposite to the active site region for linker arm immobilization. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.


Source

The source of this part is its sequence retrieved from the European Nucleotide Archive (U00096.3) along with our own modifications.

References

Shen, Y., Wu, S.Y., Rancic, V., Aggarwal, A., Qian, Y., Miyashita, S.I., Ballanyi, K., Campbell, R.E. and Dong, M., 2019. Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration. Communications biology, 2(1), pp.1-10.