Coding

Part:BBa_K3515001:Design

Designed by: Kody Klupt, Sono Khan   Group: iGEM20_Queens_Canada   (2020-05-22)
Revision as of 17:08, 22 May 2020 by Registry (Talk | contribs)

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K coil alpha helix with cysteine to be used with a E coil alpha helix for protein immobilization.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added a cysteine for immobilization (C33). Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.


Source

The source of this part is its five-heptad repeat designed by Chao et al. (https://doi.org/10.1016/S0378-4347(98)00172-8).

References