Coding
Part:BBa_K3515000:Design
Designed by: Kody Klupt, Sono Khan Group: iGEM20_Queens_Canada (2020-05-22)
E coil alpha helix with cysteine to be used with a K coil alpha helix for protein immobilization.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a cysteine for immobilization (C31). Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.
Source
The source of this part is its five-heptad repeat designed by Chao et al. (https://doi.org/10.1016/S0378-4347(98)00172-8).