Coding

Part:BBa_K2984033

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 13:32, 7 January 2020 by Ropionju (Talk | contribs)

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L1c-AR-ARS-YFP-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. L1c vector containing the arylsulfatase secretion peptide ARS1 and a mVenus fluorescent marker, flanked by AR promoter and RbcS2 terminator. The construct is designed to test for the efficiency of the secretion signal and the expression rates driven by the AR Promoter in C. reinhardtii by fluorescence intensity measurements.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 953
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 268
    Illegal PstI site found at 953
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 953
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 953
    Illegal NgoMIV site found at 1430
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisC.reinhardtii
emission528nm
excitation517 nm