Coding

Part:BBa_K2984030:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 14:23, 26 November 2019 by Ropionju (Talk | contribs) (→‎Design Notes)


L1c-Psad-ARS-YFP-SP20-Rbcs2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construct was desgined to test secretion by C. reinhardtii<i> and to be used for Level 2 MoClo Ligation

Source

The construct was created through a Level-1 ligation following the the MoClo Standard (based on Golden Gate assembly) and contains these 5 different Level-0 parts: PsaD (BBa_K2984008), ARS (BBa_K2984000), YFP (BBa_K2984017), SP20 (BBa_K2984016) and RbcS2 (BBa_K2984018).

===References===