Coding

Part:BBa_K3063987

Designed by: Ng Tsz Chun   Group: iGEM19_Hong_Kong_HKU   (2019-10-16)
Revision as of 10:54, 25 November 2019 by Kngtszchun (Talk | contribs) (Undo revision 477061 by Kngtszchun (talk))


eSIBR casette with amiRNA insertion site

eSIBR casette with amiRNA insertion site

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 585
    Illegal BglII site found at 1409
    Illegal XhoI site found at 1486
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1866
  • 1000
    COMPATIBLE WITH RFC[1000]



The part encodes three amiRNAs that silence signal transducer and activator of transcription 3 (STAT3), SRY (sex determining region Y)-box 4 (SOX4) and SRY (sex determining region Y)-box 9 (SOX9). STAT3-, SOX4- and SOX9-targeting amiRNAs in the transformed salmonella, will form dsRNAs upon binding to stemness STAT3, SOX4 and SOX9 mRNAs in tumour cells respectively. Under the aid of this particular biobrick, silencing of STAT3, SOX4 and SOX9 genes in liver cancer stem cells can be achieved simultaneously.


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