Reporter

Part:BBa_K3304016

Designed by: Platon Megagiannis   Group: iGEM19_Thessaloniki   (2019-10-16)
Revision as of 03:59, 22 October 2019 by PlatonMega (Talk | contribs)

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Amil Cp with BsaI recognition sites that insert PvuII

Amil cp used as a reporter for colony picking. we modified the standard composite functional AmilCP part with primers that insert BsaI recognition sites so that we can furthermore insert the gates into bacterial plasmids PSB1C3

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 16
    Illegal NheI site found at 39
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 740
    Illegal BsaI.rc site found at 4


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