Regulatory
PdhP

Part:BBa_K3238000

Designed by: Irving Francisco   Group: iGEM19_BUAP_Mexico   (2019-10-12)
Revision as of 03:59, 22 October 2019 by DoloresLM (Talk | contribs)

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Pyruvate dehydrogenase complex promoter, pyruvate inducible

This part is used to repress the ompW BBa_ under anaerobic conditions. Transcription of the pdh operon is controlled by two major promoters: the upstream promoter Ppdh generates a pdhR-lpdA readthrough transcript, and the internal promoter Plpd generates independent lpdA transcript. The primary pdh promoter Ppdh is negatively autoregulated by PdhR, the product of the first gene in this pdh operon

Biology

This system is composed by the response regulator ArcA and the sense kinase ArcB. When E. coli is under Anaerobic conditions ArcB has kinase activity, so it phosphorylate ArcA protein which is activated and repress the gene related with aerobic metabolism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None