Composite

Part:BBa_K2966006

Designed by: Julianne Dejoie   Group: iGEM19_Purdue   (2019-10-16)
Revision as of 03:38, 22 October 2019 by Howar216 (Talk | contribs)


PpsbA-NodC_Pf-T4 term

This part is the constitutive promoter, Pc along with the codon-optimized NodC for P. fluorescens and finally the T4 transcriptional terminator.

Characterization

T--Purdue--S3controlS4.jpg The figure above shows Pc with NodC_Pf, a negative control, and PpsbA with NodC_Pf. The negative control was used to show that the wildtype E. coli does not normally fluoresce. Pc with NodC_Pf had almost all of its cells fluoresce blue, and PpsbA with NodC_Pf had most of its cells fluoresce as well.


50px Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None