Reporter

Part:BBa_K3189015

Designed by: Jehoshua Sharma, Nathanael Wilms, Connor Gianetto-Hill   Group: iGEM19_Guelph   (2019-10-16)
Revision as of 03:28, 22 October 2019 by Cgianetto (Talk | contribs)


Tet Controlled amilCP

Tet Controlled amilCP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 146
    Illegal XhoI site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 80


test
T--Guelph--BBa_3189015a.png
Figure 1: E. coli BL21(DE3) BBa_K3189015 transformants from 11 different colonies grown in LB+tetracycline. The three wells in the top right hand corner are negative controls (E. coli without BBa_K3189015). A dark colour of variable intensity is visible at 100 ng/mL tetracycline for all of the samples.

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Categories
Parameters
None