Coding
MK

Part:BBa_K2916011

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-24)
Revision as of 03:23, 22 October 2019 by Konstantinos (Talk | contribs)


MK protein equipped with a 6x HIS affinity tag

Adenylate kinase (Myokinase) is a protein that is used in the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.


Characterization

All the data regarding the characterization of that part can be found in the composite part: BBa_K2916046 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 583
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403
    Illegal BsaI.rc site found at 433


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Parameters
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