Coding

Part:BBa_K2910002

Designed by: Victoria Sajtovich   Group: iGEM19_Toronto   (2019-10-16)
Revision as of 03:22, 22 October 2019 by AdrianaDLP (Talk | contribs)


IsPETase-ML Mut 1 with C-terminal Hexahistidine Tag

BBa_K2910002 encodes a variant of the Ideonella sakaiensis PETase, optimized for E. coli, which degrades poly(ethylene terephthalate. Using a Machine Learning Algorithm to optimize hydrophobicity content, the thermostability of the protein is predicted to be improved.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



A plot of pNB Hydrolosis at pH = 8 for BBa_K2910002 is presented below: <img src="https://parts.igem.org/File:T--Toronto--pNPB-Hydrolysis-pH-8_Tianyu-All-Conc.png">

A plot of pNB Hydrolosis at pH = 9 for BBa_K2910002 is presented below: <img src="https://parts.igem.org/File:T--Toronto--pNPB-Hydrolysis-pH-9_Tianyu-All-Conc.png">

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Parameters
None