Coding

Part:BBa_K3033009

Designed by: Philip Naderev P.Lagniton   Group: iGEM19_UM_Macau   (2019-10-12)
Revision as of 03:22, 22 October 2019 by Lagniton PN (Talk | contribs)


eGFP

Enhanced green fluorescent protein (EGFP) is a novel marker gene product, which is readily detectable using techniques of fluorescence microscopy, flow cytometry, or macroscopic imaging. EGFP allowed the practical use of GFPs in mammalian cells. EGFP is being used extensively as it offers higher-intensity emission after blue-light excitation with respect to wild-type GFP

Synthesized through IDT. This codes for the green fluorescent protein which is used to tag our fusion protein for us to see its localization


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None