Device

Part:BBa_K3304105

Designed by: Sofia Moysiadou   Group: iGEM19_Thessaloniki   (2019-10-21)
Revision as of 03:15, 22 October 2019 by Karava (Talk | contribs)


Input Gate with ELK1 sequence

This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG (part: BBa_K3304118 ) wich initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD (part: BBa_K3304108 ) and NTB (part: BBa_K3304109 ) who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB (part: BBa_K3304110 ) which constitutes the input for the downstream Join Gate (part: BBa_K3304106 ). The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.

T--Thessaloniki--animation-step1.gif T--Thessaloniki--animation-step2.gif T--Thessaloniki--results-f3.png T--Thessaloniki--results-f5.png https://2019.igem.org/File:T--Thessaloniki--results-f12.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None