Part:BBa_J364007

Test Device 4 for 2018 InterLab Study

Test Device 4 expresses GFP under the control of a constitutive promoter from the Anderson collection.

Georgia State University 2019 Characterization

A variety of culture media are available to support the bacteria commonly used in synthetic biology. Lysogeny broth (LB) is the most commonly used media in biology research. It is easy to make and compatible with most microorganisms. Terrific Broth (TB), a high nutrition solution, which has extra glycerol compared to LB media can also support E. coli and other cultures. We proposed to test the growth and expression of a foreign transgene in E. coli in two different media: LB and TB. In order to accurately measure the growth conditions, we tested both Abs600 (an indirect measure of cell density) and fluorescence data (expression of the GFP transgene) over a 6 hour time course. We chose to focus on the first 6 hours of the linear growth phase using some of the interlab test devices from the iGEM registry because we had seen some anomalies in the growth and expression curves over time. We hoped to identify the better medium to use to optimize expression of the transgene.