Part:BBa_K2992019
bgaR gene from C. perfringens
Transcriptional regulator bgaR associated with a lactose inducible system from C. perfringens
Usage and Biology
BgaR is the transcriptional regulator associated with the BgaR-BgaL lactose inducible system found naturally on the genome of C. perfringens. In our project, we use bgaR as part of the entire regulatory unit comprising bgaR, and its divergent promoter PbgaR -PbgaL with their associated 5’-UTR and RBS regions (hyperlinks and descriptions) to drive the expression of botR (BBa_K2992002) in the genome of C. sporogenes. This, in turn, leads to expression of our chosen reporter genes in an inducible fashion.
Characterisation
The Plac system is an inducible promoter system utilizing the lactose operon from C. Perfringens. This allowed us to test the maximum and minimum reporter expression range. See our results page for more information.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 368
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 368
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 368
Illegal BglII site found at 428 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 368
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 368
- 1000COMPATIBLE WITH RFC[1000]
References
Cañadas, I., Groothuis, D., Zygouropoulou, M., Rodrigues, R. and Minton, N. (2019). RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium. ACS Synthetic Biology, 8(6), pp.1379-1390.
Minton, N., Ehsaan, M., Humphreys, C., Little, G., Baker, J., Henstra, A., Liew, F., Kelly, M., Sheng, L., Schwarz, K. and Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, pp.104-112. 2019
Hartman, A., Liu, H. and Melville, S. (2010). Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression inClostridium perfringens. Applied and Environmental Microbiology, 77(2), pp.471-478.
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