Terminator

Part:BBa_K2992013

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-10)
Revision as of 02:52, 22 October 2019 by JacobGausden (Talk | contribs) (→‎Characterisation)

Usage

Tfad is a terminator sequence derived from the fad gene of C. tetani, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the pyrKDE operon of C. sporogenes, following chromosomal knock-in of botR in part BBa_K2992025, at this locus. The secondary structure for Tfad was predicted using the Mfold web tool (Zuker, 2003).

Terminator.png

Tfad from C. tetani

  • Transcriptional terminator sequence from the FAD-dependent oxidoreductase, fad, from Clostridium tetani

Secondary Structure

Mfold-K2992013-1.png

Characterisation

This basic part was used upstream of PbotR to prevent chromosomal read-through from the pyrKDE operon, upon integration of the PbotR-botR module at this locus. See our results page for more information.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 155

References

Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 31(13), pp.3406-3415.


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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Categories
Parameters
None