Device

Part:BBa_K3304000

Designed by: Platon Megagiannis   Group: iGEM19_Thessaloniki   (2019-10-14)
Revision as of 02:26, 22 October 2019 by PlatonMega (Talk | contribs)


Input Gate with NF-κB p65 consensus sequence

This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG Part:BBa_K3304005 with initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD Part:BBa_K3304006 and NTB Part:BBa_K3304007 that conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB Part:BBa_K3304006 which constitutes the input for the downstream Join Gate Part:BBa_K3304001. The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.

T--Thessaloniki--animation-step1.gif T--Thessaloniki--animation-step2.gif T--Thessaloniki--animation-step3.gif Animation describing the subsequent strand displacement Cascade steps happening in the Input Gate when no TF is bound

T--Thessaloniki--design-figure4.png Fluorescent measurements of circuit behavior after the binding of the p65 homodimer to the consensus sequence. Kinetics are compared in regards to Input Gate concentration titrations T--Thessaloniki--results-f2.png T--Thessaloniki--results-f4.png T--Thessaloniki--results-f6.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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