Composite
Part:BBa_K3117047
Designed by: Lena Schorr Group: iGEM19_FAU_Erlangen (2019-10-18)
Revision as of 02:08, 22 October 2019 by Lena Schorr (Talk | contribs)
scFv against GPA33 with SpyCatcher codon optimized for E.coli
Part BBa_K3117047 is a fusion protein of an anti-GPA33 single-chain variable fragment (scFv) and a SpyCatcher.
Usage and Biology
By connecting the variable regions of the heavy and light chain of an anti-GPA33 antibody with a short, flexible GGGGS linker (BBa_K3117028), the scFv retains it's antigen-binding ability and is much smaller than a conventional antibody. The SpyCatcher attached to the scFv belongs to the SpyTag/SpyCatcher system, of which the sequence is derived of the FbaB protein in the bacterium Streptococcus pyogenes. Once it comes into contact with its corresponding other part, the SpyTag (BBa_K3117015), they bind covalently (Hatlem et al., 2019).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 54
Illegal AgeI site found at 519 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |