Composite
Part:BBa_K3002231
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
Revision as of 02:04, 22 October 2019 by MSchlosser (Talk | contribs)
L2 hygromycin resistance + WT-PETase_His
This composite part contains a hygromycin resistance (BBa_K3002119) and the WT-PETase (BBa_K3002126) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2801
Illegal PstI site found at 3774 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2048
Illegal PstI site found at 2801
Illegal PstI site found at 3774 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1408
Illegal XhoI site found at 250 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2801
Illegal PstI site found at 3774 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2801
Illegal PstI site found at 3774
Illegal NgoMIV site found at 773
Illegal NgoMIV site found at 955
Illegal NgoMIV site found at 1326
Illegal NgoMIV site found at 2536
Illegal NgoMIV site found at 2563
Illegal NgoMIV site found at 4133 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |