Composite
Part:BBa_K3002220
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
Revision as of 01:51, 22 October 2019 by MSchlosser (Talk | contribs)
L2 spectinomycin resistance + GLE_Mut-PETase_SP20HA
This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant PETase with the secretion signal GLE (BBa_K3002110) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal PstI site found at 3503
Illegal PstI site found at 4476 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal NheI site found at 2665
Illegal PstI site found at 3503
Illegal PstI site found at 4476 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal PstI site found at 3503
Illegal PstI site found at 4476 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3238
Illegal NgoMIV site found at 3265
Illegal NgoMIV site found at 5036 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |